Speaking to the audience at her show in Santa Rosa, California this month, she said when asked where fans can see her next: "Um, no.
"This is the last time you're going to see me. After my Netflix special, I'm done." She continued: "Thank you for still caring!
"Let me catch you up on what's been going on with me since you last saw me. I got chickens."
The 66-year-old star discusses the end of her long-running programme, 'The Ellen DeGeneres Show', which ran for a decade from 2003 until 2022, but was marred by accusations of toxic behaviour by several executives in 2020, who were subsequently let go.
She quipped: "Oh yeah, and I got kicked out of show business for being mean."
Ellen then suggested she will be "kicked out for being old next". She grinned: "Old, gay and mean—the triple crown."
Ellen then joked: "I used to say, 'I don't care what people say about me.' Now, I realise I said that during the height of my popularity."
She insisted: "I am many things, but I am not mean." In April, Ellen insisted she became the "most hated person in America" at one point.
She stepped away from the spotlight after the final episode aired but returned to the public eye in April with her stand-up show at the Largo in Los Angeles, California.
Ellen told the audience: "I got kicked out of show business. There's no mean people in show business ... [I became the] most hated person in America... the hate went on for a long time and I would try to avoid looking at the news.
"The 'be kind' girl wasn't kind. That was the headline."
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'This is the last time you'll see me': Ellen DeGeneres 'done' with show business
Ellen DeGeneres will be "done" with entertaining after her Netflix special. The former talk show host has confirmed her impending retirement after 'Ellen's Last Stand...Up Tour' hits screens later this year and her latest stand-up stint